Ram 4MID® Kit (proAKAP4 biomarker)
Product ref. : 4VDX-18K7
The Ram 4MID® Kit (Ref. 4VDX-18K7) allows you to quantify proAKAP4 in samples of ram semen as a biomarker of sperm quality.
This Ram 4MID® Kit is the 1st functional test to evaluate sperm quality and fertility.
Ram 4MID® Kit description
The Ram 4MID® Kit (Ref. 4VDX-18K7) is the first test that validates objectively semen quality by quantifying a protein named proAKAP4 in samples of ram semen.
The dosage of the proAKAP4 with the Ram 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male and their ejaculates
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility
Application : for the in vitro determination of ram proAKAP4 in sperm.
For Research Use Only / Not for Diagnosis or Therapeutic Use
Reagents provided in the Ram 4MID® Kit
The Ram 4MID® Kit (Ref. 4VDX-18K7) contains all reagents and buffers required for the quantification of proAKAP4 protein in ram semen.
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Acid Stop Solution
- One vial of the Positive Control
- Two adhesive plate sealers
Please contact us contact@4biodx-breeding.com for any quotation.
Technical data of the Ram 4MID® Kit
- Name : Ram 4MID® Kit
- Product reference : 4VDX-18K7
- Specificity : Ram proAKAP4
- Analytical range : 0.9 ng to 150 ng
- Sample type : Fresh ejaculates or frozen ram semen
- 87 samples can be run simultaneously in less than 2 hours
Easy to use immunoassay / Manual and automated assay
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Contact us for technical data, price and dedicated quotation.
Benefits – Ram 4MID® Kit
The dosage of the proAKAP4 biomarker with the Ram 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male
- Qualidication of their ejaculates
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility
Overview
The Ram 4MID® Kit as the first validated test of sperm quality and fertility
The 4MID® Kit detect and quantify the proAKAP4 protein in semen (raw, diluted, fresh, chilled or frozen). ProAKAP4 is the first innovative biomarker of semen quality and of spermatozoa functionality (Sergeant et al., 2019).
ProAKAP4 is the precursor of AKAP4 that is playing a key role in flagellar structure, capacitation and sperm motility (Luconi et al., 2011 ; Turner et al., 2005). The AKAP4 gene is located on chromosome X (Sergeant et al., 2016). The precursor proAKAP4 is processed to a “mature” AKAP4 protein that will be active.
Spermatozoa without proAKAP4 / AKAP4 are abnormal, immotile and infertile (Miki et al,. 2002 ; Fang et al., 2019).
The concentration of proAKAP4 biomarker as measured with the Ram 4MID® Kit is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.
References
- Sergeant et al. (2019) Dairy Vet. Sci. J. 11(1): 803-811
- Blommaert et al. (2019) Theriogenology. 131:52-60
- Ruelle et al. (2019) Rev. Bras. Reprod. Anim. Vol43(2):472
- Blommaert et al. (2019) Rev. Bras. Reprod. Anim. Vol43(2):585
- Fang et al. (2019) Developmental Biology. In press
- Delehedde et al. (2018) Animal Reproduction Science. Vol.194:24
- Sergeant et al. (2016) Animal Reproduction Science. Vol. 169:125-126
- Luconi et al. (2011) Frontiers in Bioscience. Vol. 16:1315-1330
- Miki et al. (2002) Developmental Biology. Vol. 248:331-342
Contact our specialists for a request of quotation or any questions relating to the set up of a biomarker analysis of the quality of the semen through our contact form.
