Dog 4MID® Kit (proAKAP4 testing kit)
Product ref. : 4VDX-18K5
The Dog 4MID® Kit (Ref. 4VDX-18K5) allows to quantify proAKAP4 proteins in semen samples as an objective marker of sperm quality and male fertility in dogs (all breeds).
This Dog 4MID® Kit is the 1st functional assay to evaluate sperm quality and male fertility.
The Dog 4MID® Kit will give you a number that is correlated with sperm quality, long-lasting motily and fertility outcomes.
Application : for the in vitro determination of canine proAKAP4 in sperm (all breeds)
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Dog 4MID® Kit Description
The Dog 4MID® Kit (Ref. 4VDX-18K5) is the first assay that validates objectively semen quality by quantifying a protein called proAKAP4 in dog semen.
The dosage of the proAKAP4 biomarker with the Dog 4MID® Kit brings:
- Objective information on semen quality
- Qualification of each breeding male
- Qualification of each ejaculate and/or each straw at post-thaw quality control step
- Pertinent parameter to improve sperm cryopreservation
- Selection of semen with long-lasting motility at post-thaw
- A functional indicator correlated to fertility
Application : for the in vitro determination of dog proAKAP4 in sperm.
For Research Use Only / Not for Diagnosis or Therapeutic Use
Reagents provided in the Dog 4MID® Kit
The Dog 4MID® Kit (Ref. 4VDX-18K5) contains all reagents and buffers required for the quantification of proAKAP4 protein in dog semen from 1 to 88 analysis per microplate).
- One Microplate pre-coated with the Capture Antibody
- 7 vials of Standard (ready to use to build a standard curve)
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Acid Stop Solution
- One vial of Positive Control
- One bottle of Lysis Buffer (specific of dog semen)
- Two adhesive plate sealers
Application : in vitro quantification of canine proAKAP4 in sperm samples (fresh, chilled or frozen semen in extenders)
We have at our catalog since 2022 a BB version of our Dog 4MID® Kit (ref. 4VDX-18K5BB) to analyze from 12 to 24 semen per kit, 1 to 2 samples per strip. Upon request at : contact@4biodx-breeding.com
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Technical data of the Dog 4MID® Kit
- Name: Dog 4MID® Kit
- Product reference: 4VDX-18K5
- Specificity: Dog proAKAP4 (all breeds)
- Method: Sandwich ELISA – 96 well microplate (12 breakable strips of 8 wells)
- Analytical range: 0 ng to 150 ng
- Sample type: Ejaculate / Fresh, chilled or frozen semen in extenders / Isolated spermatozoa
- From 1 to 88 samples can be run simultaneously or individually (12 breakable strips of 8 wells per microplate/per kit)
Easy to use immunoassay
Easy to interpret / Give a number relative to sperm quality and male fertility
We have at our catalog since 2022 a BB version of our Dog 4MID® Kit (ref. 4VDX-18K5BB) to analyze from 12 to 24 semen per kit, 1 to 2 samples per strip. Upon request at : contact@4biodx-breeding.com
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Benefits – Dog 4MID® Kit
The dosage of the proAKAP4 biomarker with the Dog 4MID® Kit brings :
- Objective information on semen quality
- Qualification of each breeding male
- Qualification of each ejaculate / each straw
- Selection of semen with long-lasting motility at post-thaw
- Traceability in the quality of the semen in times
- Selection of best semen for cryopreservation
- A functional indicator of male fertility
The Dog4MID® Kit give a number that is correlated with fertility outcomes.
Do not hesitate to contact us for trial assay and quote !
We have at our catalog since 2022 a BB version of our Dog 4MID® Kit (ref. 4VDX-18K5BB) to analyze from 12 to 24 semen per kit, 1 to 2 samples per strip. Upon request at : contact@4biodx-breeding.com
Overview
The Dog 4MID® Kit as the first functional assay of semen quality and male fertility
The Dog 4MID® Kit detect and quantify the proAKAP4 protein in semen (raw, diluted, fresh, chilled or frozen / isolated spermatozoa).
ProAKAP4 is the first objective biomarker of semen quality, being a reflect of the good maturation of spermatozoa (Cui et al., 2016; Sergeant et al., 2019; Delehedde et al. 2020 ; Bastan and Akcay, 2021).
ProAKAP4 is the precursor of AKAP4 that is playing a key role in flagellar structure, capacitation and sperm motility (Luconi et al., 2011 ; Sergeant et al. 2019 ; Delehedde et al. 2019 ; Carracedo et al. 2020 ; Bastan and Akcay, 2021).
Spermatozoa without proAKAP4 / AKAP4 are abnormal, immotile and infertile (Miki et al., 2002 ; Fang et al., 2019).
Oxidative stress decrease proAKAP4 concentration in spermatozoa (Hamada et al., 2013; Nixon et al., 2019 ; Delehedde et al. 2020) and the follow up of the modulation of proAKAP4 marker is then a good indicator of environemental challenges on male fertility.
Sperm from high fertility males have higher expression of proAKAP4 than low fertility males (Peddinti et al., 2008; Ruelle et al., 2019; Sergeant et al., 2019; Singh et al., 2019; Fu et al., 2019 ; Griffin et al. 2020 ; Bastan and Akcay, 2021).
The concentration of proAKAP4 biomarker measured with the Dog 4MID® Kit is an objective indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecundation (fresh) or at post-thaw !
References
- Siena G, Milani C, Contiero B, Maenhoudt C, Robiteau G, Slimani S, Sergeant N, Tiret L, Fontbonne A (2022) Variation of ProAKAP4 concentration among different ejaculates from the same dogs. Reproduction in Domestic Animals. In press.
- Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Delehedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduction Science. Vol 247. https://doi.org/10.1016/j.anireprosci.2022.107074
- Dordas-Perpinyà M, Sergeant N, Ruelle I, Bruyas JF, Charreaux C, Michaud S, Carracedo S, Catalán J, Miró J, Delehedde M and Briand-Amirat L (2022) ProAKAP4 semen concentrations as a valuable marker protein of post-thawed semen quality and bull fertility: a retrospective study. Veterinary Sciences. Vol. 9 :224. https://doi.org/10.3390/
vetsci9050224 - Kowalczyk A, Gałeska E and Bubel A (2022) The Concentration of ProAKAP4 and Other Indicators of Cryopotential of Spermatozoa Cryopreserved in Extender with Holothuroidea Extract Addition. Animals. Vol. 12:521. https:// doi.org/10.3390/ani12040521
- Bastan and Akcay (2021) Quality assessment of frozen bull semen with the precursor A-kinase anchor protein 4 biomarker. Andrologia e14164.
- Malo C, Carracedo S, Delehedde M, Sergeant N and Skidmore L (2021) Identification of PROAKAP4 in Dromedary sperm and their correlation with monthly semen parameters. Reproduction & Fertility. Vol.2(4):268-279.
- Blommaert et al. (2021) ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media. Cryobiology. Vol. 102:133-135
- Langlade et al. (2020) Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreserv Biobank. 18(5):409-414.
- Ruelle et al. (2020) ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study. Reproduction, Fertility and Development. 32(1):145.
- Riesco et al. (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046.
- Carracedo et al. (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448
- Griffin et al. (2020) Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 160(5):695-707.
- Nixon et al. (2019) A Kinase Anchor Protein 4 is vulnerable to oxidative adduction in male germ cells. Frontiers in Cell and Developmental Biology – Vol. 7:319.
- Le Couazer et al. (2019). Expression of proAKAP4 in dog semen as promising marker of sperm quality. Reproduction in Domestic Animal. Vol 54(S3):73.
- Fang et al. (2019) Proteomics and single-cell RNA analysis of Akap4-knockout mice model confirm indispensable role of Akap4 in spermatogenesis. Developmental Biology. 454(2):118-127.
- Blommaert et al. (2019) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60.
- Delehedde et al (2019) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86.
- Delehedde et al. (2019b) ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. Vol. 2(1):13-19.
- Le Couazer et al. (2019b) ProAKAP4 as a valuable marker to assess sperm quality in dogs. Reproduction in Domestic Animals. Vol 54(2):91-92.
- Sergeant et al (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19.
- Luconi et al. (2011) Role of A-kinase anchoring proteins (AKAPs) in reproduction. Frontiers in Bioscience. Vol. 16:1315-1330.
- Miki et al. (2002) Targeted disruption of the AKAP4 gene causes defects in sperm flagellum and motility. Developmental Biology. Vol. 248:331-342.
- Teijeiro JM et al. (2012) Reproduction. Vol. 143:773-785
Complete list of references on proAKAP4/AKAP4 in male fertility upon request at contact@4biodx.com
Do not hesitate to contact us for a trial quote or for any questions relating to our innovative approach to assess semen quality and male fertility !
