Dog 4MID® Kit (proAKAP4 biomarker)
Product ref. : 4VDX-18K5
The Dog 4MID® Kit (Ref. 4VDX-18K5) allows you to quantify proAKAP4 in samples of dog semen as a biomarker of sperm quality, fertility and prolificity
This Dog 4MID® Kit is the 1st functional test to evaluate sperm quality and fertility.
The Dog 4MID® Kit will give you a number that is correlated with fertility outcomes.
Dog 4MID® Kit Description
The Dog 4MID® kit (Ref. 4VDX-18K5) is the first assay that validates objectively semen quality by quantifying a protein called proAKAP4 in dog semen.
The dosage of the proAKAP4 biomarker with the ELISA Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male
- Qualification of each ejaculate and/or each straw at post-thaw quality control step
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility
Application : for the in vitro determination of dog proAKAP4 in sperm.
For Research Use Only / Not for Diagnosis or Therapeutic Use
Reagents provided in the Dog 4MID® Kit
The Dog 4MID® Kit (Ref. 4VDX-18K5) contains all reagents and buffers required for the quantification of proAKAP4 protein in dog semen.
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Acid Stop Solution
- One vial of Positive Control
- Two adhesive plate sealers
Technical data of the Dog 4MID® Kit
- Name: Dog 4MID® Kit
- Product reference: 4VDX-18K5
- Specificity: Dog proAKAP4
- Analytical range: 0 ng to 300 ng
- Sample type: Fresh ejaculates or frozen dog semen
- From 1 to 88 samples can be run simultaneously
Easy to use immunoassay / Manual and automated assay
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Benefits – Dog 4MID® kit
The dosage of the proAKAP4 biomarker with the Dog 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male
- Qualification of their ejaculates
- Traceability in the quality of the semen
- A quality check of the sperm cryopreservation
- A functional indicator correlated to fertility and prolificity
The Bull 4MID® Kit will give you a number that is correlated with fertility outcomes.
More details in Sergent et al. 2019.
Overview
The Dog 4MID® Kit as the first validated test of sperm quality and fertility
The Dog 4MID® Kit detect and quantify the proAKAP4 protein in semen (raw, diluted, fresh, chilled or frozen).
ProAKAP4 is the first innovative biomarker of semen quality, being a reflect of the good maturation of spermatozoa (Cui et al., 2016; Sergeant et al., 2019).
ProAKAP4 is the precursor of AKAP4 that is playing a key role in flagellar structure, capacitation and sperm motility (Luconi et al., 2011 ; Turner et al., 2005). The AKAP4 gene is located on chromosome X (Sergeant et al., 2016). The precursor proAKAP4 is processed to a “mature” AKAP4 protein that will be active.
Spermatozoa without proAKAP4 / AKAP4 are abnormal, immotile and infertile (Miki et al., 2002 ; Fang et al., 2019). Also, oxidative stress decrease proAKAP4 concentration on spermatozoa (Hamada et al., 2013; Nixon et al., 2019)
Sperm from high fertility males have higher expression of proAKAP4 than low fertility males (Peddinti et al., 2008; Ruelle et al., 2019; Sergeant et al., 2019; Singh et al., 2019; Fu et al., 2019)
The concentration of proAKAP4 biomarker measured with the 4MID® Kit is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecundation
References
- Griffin et al. (2020). Reproduction. Vol. 20: 0284
- Delehedde et al. (2019) Int J Gynecol and Reprod Sci. 2(1): 13-19
- Nixon et al. (2019) Front Cell Dev Biol 7(319): 1-18
- Le Couazer et al. (2019) Reproduction in Domestic Animals. 54(2):91-92.
- Sergeant et al. (2019) Dairy Vet. Sci. J. 11(1): 803-811
- Le Couazer et al. (2019) Reproduction in Domestic Animals. 54(53):73.
- Ruelle et al. (2019) Rev. Bras. Reprod. Anim. Vol43(2):472
- Blommaert et al. (2019) Rev. Bras. Reprod. Anim. Vol43(2):585
- Dewulf et al. (2019) Dairy Vet. Sci. J. Vol. 13(3):1-7.
- Fang et al. (2019) Developmental Biology. 454(2):118-127
- Delehedde et al. (2018) Animal Reproduction Science. Vol.194:24
- Sergeant et al. (2016) Animal Reproduction Science. Vol. 169:125-126
- Teijeiro JM et al. (2012) Reproduction. Vol. 143:773-785
Contact our specialists for a request of quotation or any questions relating to the set up of a biomarker analysis of the quality of the semen through our contact form.
