Dog 4MID® Kit (proAKAP4 biomarker)
Product ref. : 4VDX-18K5
The Dog 4MID® Kit (Ref. 4VDX-18K5) allows you to quantify proAKAP4 in samples of dog semen as a biomarker of sperm quality, fertility and prolificity
This Dog 4MID® Kit is the 1st functional test to evaluate sperm quality and fertility.
The Dog 4MID® Kit will give you a number that is correlated with fertility outcomes.
Dog 4MID® Kit Description
The Dog 4MID® kit (Ref. 4VDX-18K5) is the first assay that validates objectively semen quality by quantifying a protein called proAKAP4 in dog semen.
The dosage of the proAKAP4 biomarker with the ELISA Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male
- Qualification of each ejaculate and/or each straw at post-thaw quality control step
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility
Application : for the in vitro determination of dog proAKAP4 in sperm.
For Research Use Only / Not for Diagnosis or Therapeutic Use
Reagents provided in the Dog 4MID® Kit
The Dog 4MID® Kit (Ref. 4VDX-18K5) contains all reagents and buffers required for the quantification of proAKAP4 protein in dog semen.
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Acid Stop Solution
- One vial of Positive Control
- Two adhesive plate sealers
Technical data of the Dog 4MID® Kit
- Name: Dog 4MID® Kit
- Product reference: 4VDX-18K5
- Specificity: Dog proAKAP4
- Analytical range: 0.9 ng to 60 ng
- Sample type: Fresh ejaculates or frozen dog semen
- 87 samples can be run simultaneously
Easy to use immunoassay / Manual and automated assay
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Benefits – Dog 4MID® kit
The dosage of the proAKAP4 biomarker with the Dog 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male
- Qualification of their ejaculates
- Traceability in the quality of the semen
- A quality check of the sperm cryopreservation
- A functional indicator correlated to fertility and prolificity
The Bull 4MID® Kit will give you a number that is correlated with fertility outcomes.
More details in Sergent et al. 2019.
Overview
The Dog 4MID® Kit as the first validated test of sperm quality and fertility
The Dog 4MID® Kit detect and quantify the proAKAP4 protein in semen (raw, diluted, fresh, chilled or frozen). ProAKAP4 is the first innovative biomarker of semen quality and of spermatozoa functionality (Sergeant et al.,2019).
ProAKAP4 is the precursor of AKAP4 that is playing a key role in flagellar structure, capacitation and sperm motility (Turner et al., 2005 ; Luconi et al., 2011). The AKAP4 gene is located on chromosome X (Sergeant et al., 2019). The precursor proAKAP4 is processed to a “mature” AKAP4 protein that will be active.
Spermatozoa without proAKAP4 / AKAP4 are abnormal, immotile and infertile (Miki et al,. 2002; Fang et al., 2019).
The concentration of proAKAP4 biomarker as measured with the Dog 4MID® Kit is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.
References
- Le Couazer et al. (2019a) Reproduction in Domestic Animals. In press.
- Sergeant et al. (2019) Dairy Vet. Sci. J. 11(1): 803-811
- Le Couazer et al. (2019b) Reproduction in Domestic Animals. In press.
- Ruelle et al. (2019) Rev. Bras. Reprod. Anim. Vol43(2):472
- Blommaert et al. (2019) Rev. Bras. Reprod. Anim. Vol43(2):585
- Fang et al. (2019) Developmental Biology. In press
- Le Couazer et al. (2019) Reproduction in Domestic Animals. Vol. 54(2) :91-92
- Delehedde et al. (2018) Animal Reproduction Science. Vol.194:24
- Sergeant et al. (2016) Animal Reproduction Science. Vol. 169:125-126
- Teijeiro JM et al. (2012) Reproduction. Vol. 143:773-785
Contact our specialists for a request of quotation or any questions relating to the set up of a biomarker analysis of the quality of the semen through our contact form.
