Goat 4MID® Kit (proAKAP4 biomarker)
Product ref. : 4VDX-18K9
The Goat 4MID® Kit (Ref. 4VDX-18K9) allows you to quantify proAKAP4 in samples of goat semen as a biomarker of sperm quality and fertility.
The Goat 4MID® Kit is the 1st functional test to evaluate sperm quality and fertility in goat.
The Goat 4MID® Kit will give you a number that is correlated with fertility outcomes.
Goat 4MID® Kit Description
The Goat 4MID® Kit (Ref. 4VDX-18K9) is the first test that validates objectively the semen quality by quantifying a protein named proAKAP4 in goat semen.
The dosage of the proAKAP4 biomarker with the Goat 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male
- Qualification of each ejaculate
- Qualification of each straw/straw lot at the post-thaw control step
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility
Application for the in vitro determination of goat proAKAP4 in sperm.
For Research Use Only / Not for Diagnosis or Therapeuctic Use
Reagents provided in the Goat 4MID® Kit
The Goat 4MID® kit (Ref. 4VDX-18K9) contains all reagents and buffers required for the quantification of proAKAP4 protein in goat semen.
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Stop Solution
- One positive Control
- Two adhesive plate sealers
Technical data of the Goat 4MID® Kit
- Name: Goat 4MID® Kit
- Product reference : 4VDX-18K9
- Specificity : Goat proAKAP4
- Analytical range : 0.9 ng to 60 ng
- Sample type : Fresh ejaculates or frozen goat semen
- 87 samples can be run simultaneously
Easy to use immunoassay / Manual and automated assay
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Benefits – Goat 4MID® Kit
The dosage of the proAKAP4 biomarker with the Goat 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male and their ejaculates
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility
The Goat 4MID® Kit will give you a number that is correlated with fertility outcomes.
More details in Sergeant et al. 2019.
Overview
The Goat 4MID® Kit, the first functional test to assess sperm quality and fertility
ProAKAP4 is the first innovative biomarker of semen quality and of spermatozoa functionality (Sergeant et al.,2019). ProAKAP4 is the precursor of AKAP4 that is playing a key role in flagellar structure, capacitation and sperm motility (Luconi et al., 2011 ; Turner et al., 2005). The AKAP4 gene is located on chromosome X in mammals (Sergeant et al., 2016). The proAKAP4 protein is processed to a “mature” AKAP4 protein, that is specifically localized to the fibrous sheath of the principal piece of the flagellum and is active.
Spermatozoa without proAKAP4 / AKAP4 are abnormal, immobile and infertile (Miki et al., 2002; Fang et al., 2019).
The concentration of proAKAP4 biomarker as measured with the Goat 4MID® Kit, is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.
References
- Sergeant et al. (2019) Dairy Vet. Sci. J. 11(1): 803-811
- Blommaert et al. (2019) Theriogenology. 131:52-60
- Ruelle et al. (2019) Rev. Bras. Reprod. Anim. Vol43(2):472
- Blommaert et al. (2019) Rev. Bras. Reprod. Anim. Vol43(2):585
- Fang et al. (2019) Developmental Biology. In press
- Delehedde et al. (2018) Animal Reproduction Science. Vol.194:24
- Sergeant et al. (2016) Animal Reproduction Science. Vol. 169:125-126
- Luconi et al. (2011) Frontiers in Bioscience. Vol. 16:1315-1330
- Miki et al. (2002) Developmental Biology. Vol. 248:331-342
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