Goat 4MID® Kit (proAKAP4 biomarker)
Product ref. : 4VDX-18K9
The Goat 4MID® Kit (Ref. 4VDX-18K9) allows you to quantify proAKAP4 in samples of goat semen as a biomarker of sperm quality and fertility.
The Goat 4MID® Kit is the 1st functional test to evaluate sperm quality and fertility in goat.
The Goat 4MID® Kit will give you a number that is correlated with fertility outcomes.
Goat 4MID® Kit Description
The Goat 4MID® Kit (Ref. 4VDX-18K9) is the first test that validates objectively the semen quality by quantifying a protein named proAKAP4 in goat semen.
The dosage of the proAKAP4 biomarker with the Goat 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male
- Qualification of each ejaculate
- Qualification of each straw/straw lot at the post-thaw control step
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility
Application for the in vitro determination of goat proAKAP4 in sperm.
For Research Use Only / Not for Diagnosis or Therapeuctic Use
Reagents provided in the Goat 4MID® Kit
The Goat 4MID® kit (Ref. 4VDX-18K9) contains all reagents and buffers required for the quantification of proAKAP4 protein in goat semen.
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Stop Solution
- One positive Control
- Two adhesive plate sealers
Technical data of the Goat 4MID® Kit
- Name: Goat 4MID® Kit
- Product reference : 4VDX-18K9
- Specificity : Goat proAKAP4
- Analytical range : 0.9 ng to 60 ng
- Sample type : Fresh ejaculates or frozen goat semen
- 88 samples can be run simultaneously
Easy to use immunoassay / Manual and automated assay
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Benefits – Goat 4MID® Kit
The dosage of the proAKAP4 biomarker with the Goat 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male and their ejaculates
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility
The Goat 4MID® Kit will give you a number that is correlated with fertility outcomes.
More details in Sergeant et al. 2019.
Overview
The Goat 4MID® Kit as the first validated test of sperm quality and fertility
The Goat 4MID® Kit detect and quantify the proAKAP4 protein in semen (raw, diluted, fresh, chilled or frozen).
ProAKAP4 is the first innovative biomarker of semen quality, being a reflect of the good maturation of spermatozoa (Cui et al., 2016; Sergeant et al., 2019).
ProAKAP4 is the precursor of AKAP4 that is playing a key role in flagellar structure, capacitation and sperm motility (Luconi et al., 2011 ; Turner et al., 2005). The AKAP4 gene is located on chromosome X (Sergeant et al., 2016). The precursor proAKAP4 is processed to a “mature” AKAP4 protein that will be active.
Spermatozoa without proAKAP4 / AKAP4 are abnormal, immotile and infertile (Miki et al., 2002 ; Fang et al., 2019). Also, oxidative stress decrease proAKAP4 concentration on spermatozoa (Hamada et al., 2013; Nixon et al., 2019)
Sperm from high fertility males have higher expression of proAKAP4 than low fertility males (Peddinti et al., 2008; Ruelle et al., 2019; Sergeant et al., 2019; Singh et al., 2019; Fu et al., 2019)
The concentration of proAKAP4 biomarker measured with the 4MID® Kit is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecundation.
References
- Griffin et al. (2020). Reproduction. Vol. 20: 0284
- Delehedde et al. (2019) Int J Gynecol and Reprod Sci. 2(1): 13-19
- Nixon et al. (2019) Front Cell Dev Biol 7(319): 1-18
- Sergeant et al. (2019) Dairy Vet. Sci. J. 11(1): 803-811
- Blommaert et al. (2019) Theriogenology. 131:52-60
- Ruelle et al. (2019) Rev. Bras. Reprod. Anim. Vol43(2):472
- Blommaert et al. (2019) Rev. Bras. Reprod. Anim. Vol43(2):585
- Dewulf et al. (2019) Dairy Vet. Sci. J. Vol. 13(3):1-7.
- Fang et al. (2019) Developmental Biology. Vol. 454(2):118-127
- Delehedde et al. (2018) Animal Reproduction Science. Vol.194:24
- Sergeant et al. (2016) Animal Reproduction Science. Vol. 169:125-126
- Luconi et al. (2011) Frontiers in Bioscience. Vol. 16:1315-1330
- Miki et al. (2002) Developmental Biology. Vol. 248:331-342
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