Bull 4MID® Kit (proAKAP4 biomarker)
Product ref. : 4VDX-18K4
The Bull 4MID® Kit (Ref. 4VDX-18K4) allows you to quantify proAKAP4 in bull semen as a biomarker of sperm quality and fertility.
The Bull 4MID® Kit is the 1st functional test to evaluate sperm quality.
The Bull 4MID® Kit will give you a number that is correlated with fertility outcomes.
Bull 4MID® Kit Description
The Bull 4MID® Kit (Ref. 4VDX-18K4) is the test that validate objectively semen quality by quantifying a protein biomarker named proAKAP4 in bull semen.
The dosage of the proAKAP4 biomarker with the Bull 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male
- Qualification of each ejaculate
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility
Application : for the in vitro determination of bull proAKAP4 in sperm.
For Research Use Only / Not for Diagnosis or Therapeutic Use
Reagents provided in the Bull 4MID® Kit
The Bull 4MID® Kit (Ref. 4VDX-18K4) contains all reagents and buffers required for the quantification of proAKAP4 protein in bull semen :
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Acid Stop Solution
- Two adhesive plate sealers
Technical Data of the Bull 4MID® Kit
- Name: Bull 4MID® Kit
- Product reference: 4VDX-18K4
- Specificity: Bull proAKAP4
- Analytical range: 0 ng to 300 ng
- Sample type: Fresh ejaculates, semen in extenders or frozen bull semen (straws)
- De 1 jusqu´a 88 samples can be run simultaneously
Easy to use immunoassay / Manual and automated assay
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Benefits – Bull 4MID® Kit
In bulls, quantifying proAKAP4 by the Bull 4MID® Kit method is:
- Indicative of the ejaculate quality
- An indicator of the sperm motility reserve
- An indicator of the quality of frozen straws
- A support tool for the classification of male breeder for fertility
- A monitoring tool of bull career (puberty, reform)
The Bull 4MID® Kit will give you a number that is correlated with fertility outcomes.
More details in Sergeant et al. 2019.
Overview
The Bull 4MID® Kit as the first validated test of sperm quality and fertility
The Bull 4MID® Kit detect and quantify the proAKAP4 protein in semen (raw, diluted, fresh, chilled or frozen).
ProAKAP4 is the first innovative biomarker of semen quality, being a reflect of the good maturation of spermatozoa (Cui et al., 2016; Sergeant et al., 2019).
ProAKAP4 is the precursor of AKAP4 that is playing a key role in flagellar structure, capacitation and sperm motility (Luconi et al., 2011 ; Turner et al., 2005). The AKAP4 gene is located on chromosome X (Sergeant et al., 2016). The precursor proAKAP4 is processed to a “mature” AKAP4 protein that will be active.
Spermatozoa without proAKAP4 / AKAP4 are abnormal, immotile and infertile (Miki et al., 2002 ; Fang et al., 2019). Also, oxidative stress decrease proAKAP4 concentration on spermatozoa (Hamada et al., 2013; Nixon et al., 2019)
Sperm from high fertility bulls have higher expression of proAKAP4 than low fertility bulls (Peddinti et al., 2008; Ruelle et al., 2019; Sergeant et al., 2019; Singh et al., 2019; Fu et al., 2019)
The concentration of proAKAP4 biomarker measured with the Bull 4MID® Kit is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecundation
References
- Griffin et al. (2020). Reproduction. Vol. 20: 0284
- Wu S et al. (2020) PLoS ONE.15(2):1-19
- Ruelle et al. (2020) Reproduction, Fertility and Development 2020, 32(1):145
- Delehedde et al. (2019) Int J Gynecol and Reprod Sci. 2(1): 13-19
- Nixon et al. (2019) Front Cell Dev Biol 7(319): 1-18
- Singh et al. (2019) Theriogenology. 129:130-145
- Sergeant et al. (2019) Dairy Vet. Sci. J. 11(1): 803-811
- Dewulf et al. (2019) Dairy Vet. Sci. J. Vol. 13(3):1-7.
- Fang et al. (2019) Developmental Biology. Vol. 454(2):118-127
- Ruelle et al. (2019) Rev. Bras. Reprod. Anim. Vol.43(2):472.
- Fu et al. (2019) Theriogenology. 134:74-82
- Delehedde et al. (2018) Animal Reproduction Science. Vol.194:24
- Légaré et al. (2017) Molecular Human Reproduction. Vol.1:1-15
- Sergeant et al. (2016) Animal Reproduction Science. Vol.169: 125-126
- Jagan Mojanarao G et al. (2011) Animal Reproduction Science. Vol 123:40-47
- Peddinti et al. (2008) BMC Systems Biology. Vol.2:19
- Fiedler et al. (2008) Biology of Reproduction. Vol. 78: 184-192
- Miki et al. (2002) Developmental Biology. Vol. 248: 331-342
- Moss et al. (1999) Biology of Reproduction. Vol.61:335-342
Contact our specialists through our contact form to request of quotation or if you have any questions or to set up a 4MID® trial analysis of your breeder semens.
