Bull 4MID® Kit (proAKAP4 biomarker)
Product ref. : 4VDX-18K4
The Bull 4MID® Kit (Ref. 4VDX-18K4) allows you to quantify proAKAP4 in bull semen as a biomarker of sperm quality and fertility.
The Bull 4MID® Kit is the 1st functional test to evaluate sperm quality.
The Bull 4MID® Kit will give you a number that is correlated with fertility outcomes.
Bull 4MID® Kit Description
The Bull 4MID® Kit (Ref. 4VDX-18K4) is the test that validate objectively semen quality by quantifying a protein biomarker named proAKAP4 in bull semen.
The dosage of the proAKAP4 biomarker with the Bull 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male
- Qualification of each ejaculate
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility
Application : for the in vitro determination of bull proAKAP4 in sperm.
For Research Use Only / Not for Diagnosis or Therapeutic Use
Reagents provided in the Bull 4MID® Kit
The Bull 4MID® Kit (Ref. 4VDX-18K4) contains all reagents and buffers required for the quantification of proAKAP4 protein in bull semen :
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Acid Stop Solution
- Two adhesive plate sealers
Technical Data of the Bull 4MID® Kit
- Name: Bull 4MID® Kit
- Product reference: 4VDX-18K4
- Specificity: Bull proAKAP4
- Analytical range: 0.9 ng to 60 ng
- Sample type: Fresh ejaculates, semen in extenders or frozen bull semen (straws)
- 87 samples can be run in less than two hours
Easy to use immunoassay / Manual and automated assay
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Benefits – Bull 4MID® Kit
In bulls, quantifying proAKAP4 by the Bull 4MID® Kit method is:
- Indicative of the ejaculate quality
- An indicator of the sperm motility reserve
- An indicator of the quality of frozen straws
- A support tool for the classification of male breeder for fertility
- A monitoring tool of bull career (puberty, reform)
The Bull 4MID® Kit will give you a number that is correlated with fertility outcomes.
More details in Sergeant et al. 2019.
Overview
The Bull 4MID® Kit as the first validated test of sperm quality and fertility
The Bull 4MID® Kit detect and quantify the proAKAP4 protein in semen (raw, diluted, fresh, chilled or frozen). ProAKAP4 is the first innovative biomarker of semen quality and of spermatozoa functionality (Sergeant et al., 2019). ProAKAP4 is the precursor of AKAP4 that is playing a key role in flagellar structure, capacitation and sperm motility (Luconi et al., 2011 ; Turner et al., 2005). The AKAP4 gene is located on chromosome X (Sergeant et al., 2016). The precursor proAKAP4 is processed to a “mature” AKAP4 protein that will be active.
Spermatozoa without proAKAP4 / AKAP4 are abnormal, immotile and infertile (Miki et al., 2002 ; Fang et al., 2019). Sperm from high fertility bulls have higher expression of proAKAP4 than low fertility bulls (Peddinti et al., 2008)
The concentration of proAKAP4 biomarker as measured with the 4MID® Kit is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.
References
- Singh et al. (2019) Theriogenology. 129:130-145
- Sergeant et al. (2019) Dairy Vet. Sci. J. 11(1): 803-811
- Ruelle et al. (2019) Animal Reproduction. In press.
- Fu et al. (2019) Theriogenology. 134:74-82
- Delehedde et al. (2018) Animal Reproduction Science. Vol.194:24
- Légaré et al. (2017) Molecular Human Reproduction. Vol.1:1-15
- Sergeant et al. (2016) Animal Reproduction Science. Vol.169: 125-126
- Jagan Mojanarao G et al. (2011) Animal Reproduction Science. Vol 123:40-47
- Peddinti et al. (2008) BMC Systems Biology. Vol.2:19
- Fiedler et al. (2008) Biology of Reproduction. Vol. 78: 184-192
- Miki et al. (2002) Developmental Biology. Vol. 248: 331-342
- Moss et al. (1999) Biology of Reproduction. Vol.61:335-342
Contact our specialists through our contact form to request of quotation or if you have any questions or to set up a 4MID® trial analysis of your breeder semens.
